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| Kinasera-FA biochemical assay |
Binding of ARC-Fluo probe to a protein kinase (PK) leads to high fluorescence anisotropy value of the solution as the rotation of the probe is restricted by the high molecular weight of kinase and hence the emitted light is polarized. Displacement of ARC-Fluo probe from its complex with protein kinase.
Displacement of fluorescent probe ARC-Photo-1 from its complex with PKA by various competitors [ARC-902 (?), H89 (?), ARC-341(?), ATP (?) and ATP + 10 mM Mg(OAc)2 (?)] (assay in a 384-well microplate, volume 20 microL). (NAC - normalized anisotropy change).
Kinasera-FA assay is applicable with a wide range of instruments (fluorescent spectrometers and plate-readers) equipped with polarization filters.